Astana Biomed Group, an authorized Abcam distributor in Central Asia

Feline IL-2 ELISA Kit (ab273227)

Key features and details

  • Sensitivity: 25 pg/ml
  • Range: 24.58 pg/ml - 6000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Cat

Overview

  • Product name

    Feline IL-2 ELISA Kit
    See all IL-2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    25 pg/ml

  • Range

    24.58 pg/ml - 6000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 112.5 84% - 149%
    Plasma 110.4 92% - 148%
    Cell culture media 118.9 92% - 147%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Cat

  • Product overview

    Feline IL-2 ELISA Kit ELISA Kit (ab273227) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-2 ELISA Kit in serum (Feline IL-2 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.


    This assay employs an antibody specific for Feline IL-2 ELISA Kit coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Feline IL-2 ELISA Kit antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Anti-Feline IL-2 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Feline IL-2 Antibody 2 vials
    Feline IL-2 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

  • Sequence similarities

    Belongs to the IL-2 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all

  • Database links

    Images

    • Feline IL-2 ELISA Kit standard curve
      Feline IL-2 ELISA Kit standard curve

      This standard curve is for demonstration only. A standard curve must be run with each assay.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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