Feline IL-2 ELISA Kit (ab273227)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 24.58 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Feline IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
25 pg/ml -
Range
24.58 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 112.5 84% - 149% Plasma 110.4 92% - 148% Cell culture media 118.9 92% - 147% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Feline IL-2 ELISA Kit ELISA Kit (ab273227) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-2 ELISA Kit in serum (Feline IL-2 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Feline IL-2 ELISA Kit coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Feline IL-2 ELISA Kit antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Feline IL-2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Feline IL-2 Antibody 2 vials Feline IL-2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 751114 Cat
- SwissProt: Q07885 Cat
Images
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Feline IL-2 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
