Biotin Anti-GST antibody (ab71283)
Key features and details
- Biotin Goat polyclonal to GST
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-GST antibody
See all GST primary antibodies -
Description
Biotin Goat polyclonal to GST -
Host species
Goat -
Conjugation
Biotin -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined. -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Glutathione-S-Transferase [Schistosoma japonicum]
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General notes
The antibody was labelled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab71283 was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ELISA - Biotin Anti-GST antibody (ab71283)ELISA results of ab71283 tested against purified GST. Each well was coated in duplicate with 1.0 µg of GST. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.
