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Anti-GST antibody [3G10/1B3] (ab92)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-GST antibody [3G10/1B3] (ab92)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [3G10/1B3] to GST
  • Suitable for: WB, IP
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GST antibody [3G10/1B3]
    See all GST primary antibodies
  • Description

    Mouse monoclonal [3G10/1B3] to GST
  • Host species

    Mouse
  • Specificity

    Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. This is full length GST fusion protein of 425 aa (length of the fusion protein, not GST protein alone). The GST protein is the same GST protein on the pGEX vector ( Schistosoma japonicum).
    Database link: P08515

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: 100% PBS

    w/o preservative
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    3G10/1B3
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GST
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Fusion/marker protein ELISA Kits

Images

  • Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    All lanes : Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution

    Lane 1 : Non-induced GST-fusion protein expressed in E.coli
    Lane 2 : IPTG induced GST-fusion protein expressed in E.coli

    Lysates/proteins at 5 µl per lane.

    Predicted band size: 26 kDa



    10% SDS-PAGE

  • Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    Western blot - Anti-GST antibody [3G10/1B3] (ab92)
    All lanes : Anti-GST antibody [3G10/1B3] (ab92)

    Lane 1 : Total lysate
    Lane 2 : Cytoplasmic fraction
    Lane 3 : Membrane fraction
    Lane 4 : Nuclear fraction

    Predicted band size: 26 kDa



    Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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