Anti-GST antibody (ab9085)
Key features and details
- Rabbit polyclonal to GST
- Suitable for: ELISA, IP, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-GST antibody
See all GST primary antibodies -
Description
Rabbit polyclonal to GST -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified against bacterially expressed GST -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GST antibody (ab9085) This image is courtesy of an anonymous AbreviewAll lanes : Anti-GST antibody (ab9085) at 1/2000 dilution
Lane 1 : Recombinant GST protein purified from E. coli XL1-Blue
Lane 2 : Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 26,80 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Blocked with 5% Milk for 1 hour at 25°C.
