Anti-Cannabinoid Receptor II antibody (ab3561)
Key features and details
- Rabbit polyclonal to Cannabinoid Receptor II
- Suitable for: ICC, WB, IHC-P
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Cannabinoid Receptor II antibody
See all Cannabinoid Receptor II primary antibodies -
Description
Rabbit polyclonal to Cannabinoid Receptor II -
Host species
Rabbit -
Specificity
We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanRecombinant fragmentWB Human
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Immunogen
Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).
Database link: Q9QZN9 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution
Lane 1 : HT29 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Rat colon cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
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Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.
