Anti-TPP1 antibody (ab88305)
Key features and details
- Mouse polyclonal to TPP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPP1 antibody
See all TPP1 primary antibodies -
Description
Mouse polyclonal to TPP1 -
Host species
Mouse -
Specificity
This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length human TPP1 protein (NP_000382.3).
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Positive control
- WB: Human spleen tissue lysate, Jurkat cell lysate and TPP1 transfected 293T cell lysate IHC-P: human spleen tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TPP1 antibody (ab88305)Anti-TPP1 antibody (ab88305) at 1 µg/ml + human spleen tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Western blot - Anti-TPP1 antibody (ab88305)Anti-TPP1 antibody (ab88305) at 1 µg/ml + Jurkat cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
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Western blot - Anti-TPP1 antibody (ab88305)All lanes : Anti-TPP1 antibody (ab88305) at 1 µg/ml
Lane 1 : TPP1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody (ab88305)ab88305, at 3 µg/ml, staining TPP1 in formalin fixed, paraffin embedded human spleen sections by Immunohistochemistry (Immunoperoxidase).
