Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPP1 antibody [EPR16537] (ab195234)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16537] to TPP1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TPP1 antibody [EPR16537]
    See all TPP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16537] to TPP1

  • Host species

    Rabbit

  • Specificity

    This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart, HeLa and Jurkat lysates; Human placenta tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TPP1 with ab195234 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution and counter-stained with Hematoxylin.
    (inset: negative control).

    Note: Cytoplasm staining on human placenta tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)
    Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)
    All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34, 61 kDa


    Exposure time: 3 minutes


    ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.

  • Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)
    Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)
    All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34, 61 kDa


    Exposure time: 3 minutes


    ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.

  • Anti-TPP1 antibody [EPR16537] (ab195234)
    Anti-TPP1 antibody [EPR16537] (ab195234)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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