Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Thursday March 04, 2021

Anti-TPP1 antibody [EPR16537] (ab195234)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16537] to TPP1
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-TPP1 antibody [EPR16537]
See all TPP1 primary antibodies
Description

Rabbit monoclonal [EPR16537] to TPP1
Host species

Rabbit
Specificity

This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal heart, HeLa and Jurkat lysates; Human placenta tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16537
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TPP1 with ab195234 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution and counter-stained with Hematoxylin.
(inset: negative control).

Note: Cytoplasm staining on human placenta tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)

All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/10000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 34, 61 kDa

Exposure time: 3 minutes

ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.
Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)

All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 34, 61 kDa

Exposure time: 3 minutes

ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.
Anti-TPP1 antibody [EPR16537] (ab195234)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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