Anti-SPARC antibody (ab203284)
Key features and details
- Rabbit polyclonal to SPARC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SPARC antibody
See all SPARC primary antibodies -
Description
Rabbit polyclonal to SPARC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SPARC aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P09486 -
Positive control
- IHC-P: Human lung carcinoma and colon carcinoma tissues; rat brain and mouse kidney tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-SPARC antibody (ab203284)Anti-SPARC antibody (ab203284) at 1/1000 dilution + A549 cell lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution
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Western blot - Anti-SPARC antibody (ab203284)Anti-SPARC antibody (ab203284) at 1/300 dilution + Mouse brain lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody (ab203284)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue labeling SPARC with ab203284 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody (ab203284)Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling SPARC with ab203284 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution and DAPI staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody (ab203284)Immunohistochemical analysis of formalin-fixed paraffin-embedded human colon carcinoma tissue labeling SPARC with ab203284 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody (ab203284)Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse kidney tissue labeling SPARC with ab203284 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody (ab203284)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue labeling SPARC with ab203284 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
