Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)
![Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)](https://www.abcam.com/ps/products/271/ab271980/Images/ab271980-2-ab207743270810antisparcantibodyepr20121flowcytometry.jpg "Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20121] to SPARC - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SPARC antibody [EPR20121] - BSA and Azide free
See all SPARC primary antibodies -
Description
Rabbit monoclonal [EPR20121] to SPARC - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y and A-375 whole cell lysates; Human bone giant cell tumor lysate; Human serum and brain lysate. Flow Cyt: A-375 cells. IP: A-375 whole cell lysate.
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General notes
ab271980 is the carrier-free version of ab207743. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20121 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)
Flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling SPARC with ab207743 at 1/70 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207743). -
![Immunoprecipitation - Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)](https://www.abcam.com/ps/products/271/ab271980/Images/ab271980-1-ab207743270809antisparcantibodyepr20121immunoprecipitation.jpg)
Immunoprecipitation - Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)SPARC was immunoprecipitated from 0.35 mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab207743 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207743 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: A-375 whole cell lysate, 10µg (Input).
Lane 2: ab207743 IP in A-375 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207743 in A-375 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207743). -
![Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)](https://www.abcam.com/ps/products/271/ab271980/Images/ab271980-3-benefits-of-recombinant-antibodies.png)
Anti-SPARC antibody [EPR20121] - BSA and Azide free (ab271980)
