Anti-RED1 antibody (ab89436)
Key features and details
- Mouse polyclonal to RED1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RED1 antibody
See all RED1 primary antibodies -
Description
Mouse polyclonal to RED1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length protein corresponding to amino acids 1-741 of Human RED1 (NP_056648.1).
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Positive control
- Human kidney tissue lysate, A431 cell lysate, RED1 transfected 293T cell line lysate and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RED1 antibody (ab89436)Anti-RED1 antibody (ab89436) at 1 µg/ml + Human kidney lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 81 kDa
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Western blot - Anti-RED1 antibody (ab89436)Anti-RED1 antibody (ab89436) at 1 µg/ml + A431 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
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Western blot - Anti-RED1 antibody (ab89436)All lanes : Anti-RED1 antibody (ab89436) at 1 µg/ml
Lane 1 : RED1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-RED1 antibody (ab89436)ab89436 at 10 µg/ml staining RED1 in HeLa cells by Immunofluorescence.
