Anti-PGLS antibody (ab229980)
Key features and details
- Rabbit polyclonal to PGLS
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PGLS antibody
See all PGLS primary antibodies -
Description
Rabbit polyclonal to PGLS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant full length protein corresponding to Human PGLS aa 1 to the C-terminus.
Database link: O95336 -
Positive control
- WB: Mouse liver and brain lysates; U-251 MG, THP-1, HepG2 and HeLa whole cell lysates. IHC-P: Human thyroid and tonsil tissues. IP: U-251 MG whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PGLS antibody (ab229980)All lanes : Anti-PGLS antibody (ab229980) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28,52 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (ab229980)Paraffin-embedded human thyroid tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (ab229980)Paraffin-embedded human tonsil tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-PGLS antibody (ab229980)PGLS was immunoprecipitated from 500 µg U-251 MG (human brain glioma cell line) whole cell lysate with 4 µg ab229980.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in U-251 MG whole cell lysate.
Lane 2: ab229980 IP in U-251 MG whole cell lysate.
Lane 3: U-251 MG whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
