Anti-PGLS antibody (ab229872)
Key features and details
- Rabbit polyclonal to PGLS
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PGLS antibody
See all PGLS primary antibodies -
Description
Rabbit polyclonal to PGLS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant full length protein corresponding to Human PGLS aa 1 to the C-terminus.
Database link: O95336 -
Positive control
- WB: Mouse heart, brain and liver tissue lysates. U251, THP-1, HepG2 and HeLa whole cell lysates. IHC-P: Human thyroid and tonsil tissues. IP: PGLS in U251 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PGLS antibody (ab229872)All lanes : Anti-PGLS antibody (ab229872) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : U251 whole cell lysate
Lane 5 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (ab229872)Paraffin-embedded human thyroid tissue stained for PGLS with ab229872 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (ab229872)Paraffin-embedded human tonsil tissue stained for PGLS with ab229872 at a 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-PGLS antibody (ab229872)PGLS was immunoprecipitated from 500 µg U251 whole cell extracts using 4 µg of ab229872.
Lane 1: Control IgG IP in U251 whole cell extracts.
Lane 2: ab229872 in U251 whole cell extracts.
Lane 3: PGLS in U251 whole cell extracts (Input).
For WB an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000).
