Human Sorbitol Dehydrogenase ELISA Kit (ab233613)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 60 pg/ml
- Range: 0.39 ng/ml - 25 ng/ml
- Sample type: Cell culture extracts, Cit plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Sorbitol Dehydrogenase ELISA Kit
See all Sorbitol Dehydrogenase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Jurkat cells 5 2.3% Inter-assay Sample n Mean SD CV% Jurkat cells 3 7.9% -
Sample type
Urine, Serum, Cell culture extracts, Tissue Extracts, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
60 pg/ml -
Range
0.39 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 107 102% - 113% Serum 95 92% - 97% Cell culture extracts 99 89% - 109% Tissue Culture Media 103 92% - 114% Cit plasma 86 81% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Sorbitol Dehydrogenase ELISA Kit (ab233613) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Sorbitol Dehydrogenase protein in cit plasma, serum, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Sorbitol Dehydrogenase with 60 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Sorbitol Dehydrogenase Capture Antibody 1 x 600µl 10X Human Sorbitol Dehydrogenase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Sorbitol Dehydrogenase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. -
Tissue specificity
Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level). -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. -
Cellular localization
Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. - Information by UniProt
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Alternative names
- DHSO_HUMAN
- L iditol 2 dehydrogenase
- L-iditol 2-dehydrogenase
see all -
Database links
- Entrez Gene: 6652 Human
- Omim: 182500 Human
- SwissProt: Q00796 Human
- Unigene: 878 Human
Images
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Other - Human Sorbitol Dehydrogenase ELISA Kit (ab233613)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Sorbitol Dehydrogenase standard curve in Sample Diluent NS.The Sorbitol Dehydrogenase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Sorbitol Dehydrogenase standard curve in 1X Cell Extraction Buffer.The Sorbitol Dehydrogenase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Sorbitol Dehydrogenase in human serum, plasma and urine samples.The concentrations of Sorbitol Dehydrogenase were measured in duplicates, interpolated from the Sorbitol Dehydrogenase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 25%, and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Sorbitol Dehydrogenase in human serum, and plasma samples.The concentrations of Sorbitol Dehydrogenase were measured in duplicates, interpolated from the Sorbitol Dehydrogenase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Sorbitol Dehydrogenase concentration was determined to be 19 ng/mL in serum and 16 ng/mL in plasma(citrate).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated concentrations of native Sorbitol Dehydrogenase in human Jurkat cell extract and human liver tissue extract samples.
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Interpolated concentrations of native Sorbitol Dehydrogenase in human Jurkat cell extract and human liver tissue extract samples.The concentrations of Sorbitol Dehydrogenase were measured in duplicate and interpolated from the Sorbitol Dehydrogenase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Sorbitol Dehydrogenase concentration was determined to be 0.06 ng/µg in Jurkat cell extract and 3.3 ng/µg in liver tissue extract.
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Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (ab233613)To learn more about the advantages of recombinant antibodies see here.
