Anti-Peroxiredoxin-SO3 antibody (ab16830)
Key features and details
- Rabbit polyclonal to Peroxiredoxin-SO3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin-SO3 antibody -
Description
Rabbit polyclonal to Peroxiredoxin-SO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic sulfonylated peptide (Human) conjugated to KLH, corresponding to Prx I-IV active site.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.09% Sodium chloride -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Peroxiredoxin-SO3 antibody (ab16830)
Upper blot - Prx-SO3 antibody (ab16830) at 1/2000
Lower blot - Prx1 antibody as a loading controlLane 1: H2O2 untreated HeLa cell lysate
Lane 2: H2O2 treated HeLa cell lysate
Lane 3: Reduced recombinant Prx1
Lane 4: Oxidized recombinant Prx1 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin-SO3 antibody (ab16830)IHC image of ab16830 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16830,1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.