Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [AT4E10] to GARS
Suitable for: WB, ICC
Reacts with: Human

Product name

Anti-GARS antibody [AT4E10]
See all GARS primary antibodies
Description

Mouse monoclonal [AT4E10] to GARS
Host species

Mouse
Tested applications

Suitable for: WB, ICCmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human GARS aa 1-300.
Database link: P41250
Positive control
- WB: HeLa, HEK-293T, Jurkat, U87MG, A431 cell lysate. ICC: HeLa cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 0.75% Sodium chloride, 0.02% Potassium chloride, 0.13% Dibasic monohydrogen sodium phosphate, 0.02% Monobasic dihydrogen potassium phosphate, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

AT4E10
Isotype

IgG1
Light chain type

kappa

Western blot - Anti-GARS antibody (ab282154)

All lanes : Anti-GARS antibody [AT4E10] (ab282154) at 1/1000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 5 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat anti-Mouse secondary antibody HRP-conjugated

Predicted band size: 83 kDa

SDS-PAGE gel was transferred to PVDF membrane.

ECL detection system.
Immunocytochemistry - Anti-GARS antibody (ab282154)

Immunocytochemical analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling GARS with ab282154 at 1/100 dilution followed by Alexa Fluor 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).
Anti-GARS antibody [AT4E10] (ab282154)

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