Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GARS antibody [AT4E10] (ab282154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AT4E10] to GARS
  • Suitable for: WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-GARS antibody [AT4E10]
    See all GARS primary antibodies

  • Description

    Mouse monoclonal [AT4E10] to GARS

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human GARS aa 1-300.
    Database link: P41250

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, U87MG, A431 cell lysate. ICC: HeLa cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 0.75% Sodium chloride, 0.02% Potassium chloride, 0.13% Dibasic monohydrogen sodium phosphate, 0.02% Monobasic dihydrogen potassium phosphate, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    AT4E10

  • Isotype

    IgG1

  • Light chain type

    kappa

Images

  • Western blot - Anti-GARS antibody (ab282154)
    Western blot - Anti-GARS antibody (ab282154)
    All lanes : Anti-GARS antibody [AT4E10] (ab282154) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 5 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse secondary antibody HRP-conjugated

    Predicted band size: 83 kDa



    SDS-PAGE gel was transferred to PVDF membrane.

    ECL detection system.

  • Immunocytochemistry - Anti-GARS antibody (ab282154)
    Immunocytochemistry - Anti-GARS antibody (ab282154)
    Immunocytochemical analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling GARS with ab282154 at 1/100 dilution followed by Alexa Fluor 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).
  • Anti-GARS antibody [AT4E10] (ab282154)
    Anti-GARS antibody [AT4E10] (ab282154)

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