Anti-GRP94 antibody (ab13509)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Rabbit polyclonal to GRP94 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse GRP94 aa 787-802 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:EEEEETEKESTEKDEL
Database link: P08113
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GRP94 antibody (ab13509)All lanes : Anti-GRP94 antibody (ab13509) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from rat brain
Lane 3 : Lysates prepared from mouse brain
Lane 4 : Lysates prepared from rat liver microsomes
Lane 5 : Lysates prepared from mouse liver microsomes
Lane 6 : Lysates prepared from human liver microsomes
Predicted band size: 94 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab13509)ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-GRP94 antibody (ab13509)
