Recombinant Human B3GALTL protein (His tag) (ab276604)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human B3GALTL protein (His tag) -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
SEDTKKEVKQ SQDLEKSGIS RKNDIDLKGI VFVIQSQSN SFHAKRAEQL KKSILKQAAD LTQELPSVLL LHQLAKQEGA WTILPLLPHF SVTYSRNSS WIFFCEEETR IQIPKLLETL RRYDPSKEWF LGKALHDEEA TIIHHYAFSE NPTVFKYPD FAAGWALSIP LVNKLTKRLK SESLKSDFTI DLKHEIALYI WDKGGGPPLT PVPEFCTND VDFYCATTFH SFLPLCRKPV KKKDIFVAVK TCKKFHGDRI PIVKQTWESQ ASLIEYYSD YTENSIPTVD LGIPNTDRGH CGKTFAILER FLNRSQDKTA WLVIVDDDTL ISISRLQHL LSCYDSGEPV FLGERYGYGL GTGGYSYITG GGGMVFSREA VRRLLASKCR CYSNDAPDD MVLGMCFSGL GIPVTHSPLF HQARPVDYPK DYLSHQVPIS FHKHWNIDPV KVYFTWLAP SDEDKARQET QKGF -
Predicted molecular weight
55 kDa including tags -
Molecular weight information
The recombinant human B3GALTL comprises 486 amino acids and has a predicted molecular mass of 55.4 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions due to glycosylation. -
Amino acids
29 to 494 -
Tags
His tag N-Terminus -
Additional sequence information
Predicted N-terminus: His.
Specifications
Our Abpromise guarantee covers the use of ab276604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- B3GlcT
- B3GTL
- Beta 1,3 galactosyltransferase like
see all -
Relevance
B3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1; MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2; MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008). -
Cellular localization
Endoplasmic reticulum membrane; Single pass type II membrane protein.
Images
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SDS-PAGE - Recombinant Human B3GALTL protein (ab276604)SDS-PAGE analysis of ab276604
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276604 has not yet been referenced specifically in any publications.
Preparation and Storage
- B3GlcT
- B3GTL
- Beta 1,3 galactosyltransferase like
Images
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SDS-PAGE - Recombinant Human B3GALTL protein (ab276604)SDS-PAGE analysis of ab276604