Anti-Calcitonin receptor/CT-R antibody (ab83297)
Key features and details
- Rabbit polyclonal to Calcitonin receptor/CT-R
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Calcitonin receptor/CT-R antibody
See all Calcitonin receptor/CT-R primary antibodies -
Description
Rabbit polyclonal to Calcitonin receptor/CT-R -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Rat Calcitonin receptor/CT-R aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab93306) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse small intestine; Rat kidney.
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General notes
Previously labelled as Calcitonin receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12311 Mouse
- Entrez Gene: 116506 Rat
- SwissProt: Q60755 Mouse
- SwissProt: P32214 Rat
- Unigene: 4642 Mouse
- Unigene: 10062 Rat
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Alternative names
- Calcitonin receptor antibody
- Calcitonin receptor precursor antibody
- CALCR antibody
see all
Images
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Western blot - Anti-Calcitonin receptor/CT-R antibody (ab83297)All lanes : Anti-Calcitonin receptor/CT-R antibody (ab83297) at 1 µg/ml
Lane 1 : Small Intestine (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Rat Calcitonin receptor precursor has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data), explaining the bands seen around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (1)
ab83297 has been referenced in 1 publication.
- Hughes S et al. Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina. Sci Rep 7:46085 (2017). IHC-Fr ; Mouse . PubMed: 28443635
Images
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Western blot - Anti-Calcitonin receptor/CT-R antibody (ab83297)All lanes : Anti-Calcitonin receptor/CT-R antibody (ab83297) at 1 µg/ml
Lane 1 : Small Intestine (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Rat Calcitonin receptor precursor has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data), explaining the bands seen around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
