Anti-Calcitonin receptor/CT-R antibody (ab230500)
Key features and details
- Rabbit polyclonal to Calcitonin receptor/CT-R
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calcitonin receptor/CT-R antibody
See all Calcitonin receptor/CT-R primary antibodies -
Description
Rabbit polyclonal to Calcitonin receptor/CT-R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Calcitonin receptor/CT-R aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P30988 -
Positive control
- IHC-P: Human colon (vessels) tissue. ICC/IF: MCF7 cells. WB: MCF7 and A431 whole cell lysates. Flow: HL-60 cells.
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General notes
Previously labelled as Calcitonin receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calcitonin receptor/CT-R antibody (ab230500)All lanes : Anti-Calcitonin receptor/CT-R antibody (ab230500) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate without peptide (-)
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate with peptide (+)
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate without peptide (-)
Lane 4 : A431 (human epidermoid carcinoma cell line) cell lysate with peptide (+)
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : goat anti-rabbit(HRP) at 1/5000 dilution
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Flow Cytometry - Anti-Calcitonin receptor/CT-R antibody (ab230500)Flow cytometric analysis of calcitonin in HL-60 (human promyelocytic leukemia cell line) cells using ab230500 at 1/10 dilution (right histogram) compared to the negetive contolr (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin receptor/CT-R antibody (ab230500)MCF7 (human breast adenocarcinoma cell line) cells labelling calcitonin using ab230500 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in ICC/IF.
Nuclear counterstain: DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin receptor/CT-R antibody (ab230500)Formalin-fixed, paraffin-embedded human colon (vessels) tissue stained for calcitonin using ab230500 at 1/50 dilution in immunohistochemical analysis.
