Recombinant human AMPK alpha 2 + PRKAG3 + AMPK beta 2/PRKAB2 protein (Active) (ab268342)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, GST tag N-Terminus, His tag C-Terminus, GST tag N-Terminus, His tag C-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human AMPK alpha 2 + PRKAG3 + AMPK beta 2/PRKAB2 protein (Active) -
Biological activity
The specific activity of ab268342 was 58 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
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Purity
> 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQ KIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGE LFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAH MNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSC GVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQ VDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKF ECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGS FMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKA KWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNY VKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRS SFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTL AR -
Molecular weight information
SDS-PAGE molecular weight: ~92 kDa -
Amino acids
1 to 552 -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
GeneBank: NM_006252
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Amino Acid Sequence 2
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Species
Human -
Sequence
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPD SKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWS TKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTIN NLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEE RFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSV MVLSATHRYKKKYVTTLLYKPI -
Molecular weight information
SDS-PAGE molecular weight: ~62 kDa -
Amino acids
1 to 272 -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
GeneBank: NM_005399
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Amino Acid Sequence 3
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Species
Human -
Sequence
MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIR GKRRAKALRWTRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLA QADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFPATEAWECELE GLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATS SKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVL HRYYRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTL IKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQD LGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFD VIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIARE QVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA -
Molecular weight information
SDS-PAGE molecular weight: ~108 kDa -
Amino acids
1 to 489 -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
GeneBank: NM_017431
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Preparation and Storage
Images
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Functional Studies - Recombinant human AMPK alpha 2 + PRKAG3 + AMPK beta 2/PRKAB2 protein (Active) (ab268342)
The specific activity of ab268342 was 58 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
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SDS-PAGE - Recombinant human AMPK alpha 2 + PRKAG3 + AMPK beta 2/PRKAB2 protein (Active) (ab268342)SDS-PAGE analysis of ab268342.
