DoubleStain IHC Kit: M&M on Mouse tissue (BCIP & AEC) (ab183274)
Overview
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Product name
DoubleStain IHC Kit: M&M on Mouse tissue (BCIP & AEC) -
Assay type
Polymer Multiplex -
Product overview
Abcam's Doublestain IHC Kit (ab183274) is designed to use with two user-supplied mouse antibodies to detect two distinct antigens on mouse tissue or cell samples. Specimens can be frozen, paraffin embedded, or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra-sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit (ab183274) supplies the user with a primer system to enhance the two polymer enzyme conjugates; Mouse HRP (AEC) Polymer and Mouse AP Polymer with two distinct substrates/chromogens: AEC and BCIP/NBT. BCIP/NBT reacts with Mouse AP Polymer conjugate to produce a purple color. AEC chromogen reacts with Mouse HRP (AEC) Polymer conjugate to produce a red color. Abcam's Doublestain IHC Kit (ab183274) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.
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Tested applications
Suitable for: IHC-P, IHC-Frmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 12 ml 36 ml 120 ml AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 8ml AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 4ml Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml Blocker A 1 x 6ml 1 x 18ml 1 x 60ml Blocker B 1 x 6ml 1 x 18ml 1 x 60ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 4ml Mouse AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse Primer 1 x 6ml 1 x 18ml 1 x 60ml Ready to Use BCIP/NBT 1 x 7ml 1 x 18ml 1 x 60ml -
Research areas