Abcam’s DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) (ab210060) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears. 

Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The AEC chromogen (red color) is used with HRP Polymer anti-Mouse IgG and the BCIP/NBT chromogen (purple/blue color) is used with AP Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.