Abcam's DoubleStain IHC kit (ab183272) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin tissue, however, it can be also be used on frozen specimen and freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC kit supplies two polymer enzyme conjugates: Goat AP polymer and Mouse HRP polymer with two distinct substrates/chromogens, Emerald (Green) and Permanent Red (Red). Simplified steps offer a convenient protocol as the enzyme conjugates are sequentially applied to the specimen. A second advantage of this kit is that it allows the researcher to visualize two co-localized proteins and due to the color change when the chromogens overlap this can be semi-quantitative. For example, if the area of co-localization stains blue, the antigen indicated by Emerald is expressed at a higher concentration in the cell and if the color is purple, the antigen indicated by Permanent-Red is expressed at higher concentrations. Abcam's DoubleStain IHC kit is a non-biotin system avoiding endogenous biotin non-specific binding.

• Kits/ Lysates/ Other
• Kits
• IHC tools
• Multicolor chromogenic