Abcam's Doublestain IHC Kit (ab183275) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. The advantage of this kit series is that it will allow you to visualize when two proteins are co localized by producing a third color blue purple. Specimens can be frozen or paraffin embedded, or freshly prepared monolayer cell smears.

Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit (ab183275) supplies the user with primer system to enhance the two polymer enzyme conjugates; Mouse HRP Polymer and Mouse AP Polymer with two distinct substrates/chromogens, Permanent Red and Emerald. Permanent Red reacts with Mouse AP Polymer conjugate to produce a red color. Emerald chromogen reacts with Mouse HRP Polymer conjugate to produce a green color. However when the chromogens are produced in the same place the color appears blue to purple in color. Abcam's Doublestain IHC Kit (ab183275) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

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• Multicolor chromogenic