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Anti-MCL1 antibody (ab28147)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-MCL1 antibody (ab28147)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to MCL1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Dog, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MCL1 antibody
    See all MCL1 primary antibodies
  • Description

    Rabbit polyclonal to MCL1
  • Host species

    Rabbit
  • Specificity

    This antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human MCL1 aa 121-139 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    SPEEELDGYEPEPLGKRPA

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: Human HeLa cells; IHC-P: Human breast cancer tissue; WB: Hela, MDCK and RK-13 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-MCL1 antibody (ab28147)
    Western blot - Anti-MCL1 antibody (ab28147)
    All lanes : Anti-MCL1 antibody (ab28147) at 1/500 dilution

    Lane 2 : HeLa whole cell lysate
    Lane 3 : 3T3 whole cell lysate
    Lane 4 : PC-12 whole cell lysate
    Lane 5 : MDCK whole cell lysate
    Lane 6 : RK-13 whole cell lysate

    Predicted band size: 42-43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody (ab28147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody (ab28147)

    ab28147 staining MCL1 in human breast cancer tissue cells by Immunohistochemistry

  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody (ab28147)
    Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody (ab28147)

    ab28147 staining MCL1 in human HeLa cells by Immunocytochemistry/ immunofluorescence.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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