Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCL1 antibody [19C4-15] - BSA and Azide free (ab252253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [19C4-15] to MCL1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MCL1 antibody [19C4-15] - BSA and Azide free
    See all MCL1 primary antibodies

  • Description

    Rat monoclonal [19C4-15] to MCL1 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Mouse MCL1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P97287

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: SK-MEL-2, Raji, C2C12 and EL4 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252253 is the carrier-free version of ab243136. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MCL1 antibody [19C4-15] - BSA and Azide free (ab252253)
    Western blot - Anti-MCL1 antibody [19C4-15] - BSA and Azide free (ab252253)
    All lanes : Anti-MCL1 antibody [19C4-15] (ab243136) at 1/1000 dilution

    Lane 1 : SK-MEL-2 (human skin malignant melanoma) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate
    Lane 4 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

    Predicted band size: 37 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243136).

  • Anti-MCL1 antibody [19C4-15] - BSA and Azide free (ab252253)
    Anti-MCL1 antibody [19C4-15] - BSA and Azide free (ab252253)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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