Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase p110 delta antibody [EPR386]
    See all PI 3 Kinase p110 delta primary antibodies

  • Description

    Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, Raji, K562, RAW 264.7, Rat brain, Rat kidney and Rat spleen lysates. ICC/IF: RAW264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

    Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PI 3 Kinase p110 delta with Purified ab109006 at 1:150 dilution (9.8 µg/ml). Cells were fixed in 100% Methanol and permeabilized with none. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    All lanes : Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1.5 µg/ml (purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 3 : Rat spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 119 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: PIK3CD knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Jurkat whole cell lysate (40 µg)
    Lane 4: K562 whole cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109006 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab109006 was shown to specifically react with PIK3CD in wild-type HAP1 cells. No band was observed when PIK3CD knockout samples were used. Wild-type and PIK3CD knockout samples were subjected to SDS-PAGE. Ab109006 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    All lanes : Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1/1000 dilution (Unpurified)

    Lane 1 : Jurkat cell lysate

    Lane 2 : Raji cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 119 kDa

  • Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)
    Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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