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Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1653] to TNF Receptor II - Low endotoxin, Azide free
  • Suitable for: IP, IHC-P, Flow Cyt, WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free
    See all TNF Receptor II primary antibodies

  • Description

    Rabbit monoclonal [EPR1653] to TNF Receptor II - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, Flow Cyt, WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat cells; ICC: Jurkat cells; IHC-P: Human breast carcinoma tissue; WB: Jurkat, NIH/3T3 and C6 whole cell lysates.

  • General notes

    ab221921 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    Western blot - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    All lanes : Anti-TNF Receptor II antibody [EPR1653] (ab109322) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    The molecular mass observed is consistent with what has been described in the literatures (PMID: 8069916, 25609706).

  • Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    This data was developed using ab109322, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    This data was developed using ab109322, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/50 dilution (2.66 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    This data was developed using ab109322, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TNF Receptor II with Purified ab109322 at 1/150 dilution (0.89 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    This data was developed using abab109322, the same antibody clone in a different buffer formulation.
    Purified ab109322 at 1/20 dilution (0.6µg) immunoprecipitating TNF Receptor II in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab109322 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109322 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 73 kDa
  • Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)

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