Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TNF Receptor II antibody [EPR1653] (ab109322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1653] to TNF Receptor II
  • Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TNF Receptor II antibody [EPR1653]
    See all TNF Receptor II primary antibodies

  • Description

    Rabbit monoclonal [EPR1653] to TNF Receptor II

  • Host species

    Rabbit

  • Specificity

    We do not guarantee IHC-P for mouse and rat species.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat cells; ICC: Jurkat cells; IHC-P: Human breast carcinoma tissue; WB: Jurkat, NIH/3T3 and C6 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Western blot - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    All lanes : Anti-TNF Receptor II antibody [EPR1653] (ab109322) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa



    The molecular mass observed is consistent with what has been described in the literatures (PMID: 8069916, 25609706).

  • Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Immunocytochemistry - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TNF Receptor II with Purified ab109322 at 1/50 dilution (2.66 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TNF Receptor II with Purified ab109322 at 1/150 dilution (0.89 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Immunoprecipitation - Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Purified ab109322 at 1/20 dilution (0.6µg) immunoprecipitating TNF Receptor II in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab109322 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109322 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 73 kDa
  • Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    Anti-TNF Receptor II antibody [EPR1653] (ab109322)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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