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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RASGRP3 (phospho S172) antibody [EPNCIR149]
    See all RASGRP3 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human RASGRP3.

  • Positive control

    • LNCap wild type cell lysates transfected with wild type RasGRP3, (C) wild type RASGRP3 + PMA (phorbol 12-myristate 13-acetate), wild type RASGRP3 + Go6983, wild type RASGRP3 + Go6983 + PMA
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR149
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Images

  • Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)
    Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)
    All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution (purified)

    Lane 1 : LnCap wild type cell lysate transfected with RASGRP3 + Go6983
    Lane 2 : LnCap wild type cell lysate, untransfected

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)
    Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)
    All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution

    Lane 1 : LNCap wild type
    cell lysate untransfected
    Lane 2 : LNCap wild type
    cell lysate transfected with RASGRP3
    Lane 3 : LNCap wild type
    cell lysate transfected with RASGRP3 + PMA
    Lane 4 : LNCap wild type
    cell lysate transfected with RASGRP3 + Go6983
    Lane 5 : LNCap wild type
    cell lysate transfected with RASGRP3 + Go6983 + PMA
    Lane 6 : LNCap wild type
    cell lysate transfected with s172A mutant RASGRP3
    Lane 7 : LNCap wild type
    cell lysate transfected with
    s172A mutant RASGRP3 + PMA
    Lane 8 : LNCap wild type
    cell lysate transfected with s172A mutant RASGRP3 + Go6983
    Lane 9 : LNCap wild type
    cell lysate transfected with s172A mutant RASGRP3 + Go6983 + PMA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 78 kDa

  • Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)
    Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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