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Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)

Key features and details

  • Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA
  • Suitable for: IHC-P, Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Carcino Embryonic Antigen CEA antibody [26/3/13]
    See all Carcino Embryonic Antigen CEA primary antibodies

  • Description

    Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA

  • Host species

    Mouse

  • Specificity

    This antibody recognises exclusively CEACAM 5 transiently expressed on the cell surface of transfected BOSC cells. It can be used to distinguish CEACAM 5 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM7), CEACAM 6 (NCA/CD66c), CEACAM 7 (CGM2), CEACAM 8 (CGM6/CD66b)and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members. This antibody was included and characterized in the studies from the VIth Leucocyte Typing Workshop (Grunert F et al. 1994)

  • Tested applications

    Suitable for: IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Grunert F, et al. 1985)

  • Positive control

    • IHC-P: human normal colon tissue sections

  • General notes

    Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)
    IHC image of Carcino Embryonic Antigen CEA staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4451, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Sandwich ELISA - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)
    Sandwich ELISA - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)
    Standard Curve for Carcino Embryonic Antigen CEA dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451) at 0.2ug/ml and Detector Antibody Rabbit polyclonal to Carcino Embryonic Antigen CEA (ab15987) at 0.5ug/ml

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