HRP Anti-BNIP3 antibody [ANa40] (ab196707)
![HRP Anti-BNIP3 antibody [ANa40] (ab196707)](https://www.abcam.com/ps/products/196/ab196707/Images/ab196707-238287-WBab1967071.jpg "HRP Anti-BNIP3 antibody [ANa40] (ab196707)")
Key features and details
- HRP Mouse monoclonal [ANa40] to BNIP3
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-BNIP3 antibody [ANa40]
See all BNIP3 primary antibodies -
Description
HRP Mouse monoclonal [ANa40] to BNIP3 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human BNIP3 aa 1 to the C-terminus.
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Epitope
The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule. -
Positive control
- WB: Human skeletal muscle tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
ANa40 -
Myeloma
unknown -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-BNIP3 antibody [ANa40] (ab196707)HRP Anti-BNIP3 antibody [ANa40] (ab196707) at 1/5000 dilution + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21.5 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196707 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
