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Immunology Adaptive Immunity T Cells CD

Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)

Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7276] to CD4 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD4 antibody [EPR7276] - BSA and Azide free
    See all CD4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7276] to CD4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD4 aa 1-250 (extracellular). The exact sequence is proprietary.
    Database link: P01730

  • General notes

    Ab248580 is the carrier-free version of ab133622. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248580 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7276
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Images

  • Western blot - Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)
    Western blot - Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)
    All lanes : Anti-CD4 antibody [EPR7276] (ab133622) at 1/1000 dilution

    Lane 1 : Human fetal thymus lysate
    Lane 2 : Human lymph node lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    This data was developed using ab133622, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)
    Flow Cytometry - Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)
    This data was developed using ab133622, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of THP-1 cells with ab133622 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
  • Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)
    Anti-CD4 antibody [EPR7276] - BSA and Azide free (ab248580)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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