Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)

Key features and details

Mouse monoclonal [C86/1146] to CD86 - BSA and Azide free
Suitable for: WB, ICC, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-CD86 antibody [C86/1146] - BSA and Azide free
See all CD86 primary antibodies
Description

Mouse monoclonal [C86/1146] to CD86 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: WB, ICC, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Recombinant full length protein corresponding to Human CD86.
Database link: P42081
Positive control
- WB: Daudi cell lysate; ICC: Raji cells; IHC-P: Human melanoma tissue.
General notes

ab213045 is a carrier free version of ab220188. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

ab22188 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
Clonality

Monoclonal
Clone number

C86/1146
Isotype

IgG1
Light chain type

kappa
Research areas

Immunocytochemistry - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)

This image was produced using ab220188, the same clone but in a different formulation.

Confocal Immunofluorescence image of Raji cells labeling CD86 with ab220188 followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody (ab213045)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling CD86 with ab220188 at 4 µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220188).
Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)

Anti-CD86 antibody [C86/1146] (ab220188) at 2 µg/ml + Daudi (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 38 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

CD86 is observed as a 70-kDa glycoprotein in Western blot.

This image was produced using ab220188, the same clone but in a different formulation.

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