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Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)

Key features and details

  • Mouse monoclonal [C86/1146] to CD86 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD86 antibody [C86/1146] - BSA and Azide free
    See all CD86 primary antibodies

  • Description

    Mouse monoclonal [C86/1146] to CD86 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Human CD86.
    Database link: P42081

  • Positive control

    • WB: Daudi cell lysate; ICC: Raji cells; IHC-P: Human melanoma tissue.

  • General notes

    ab213045 is a carrier free version of ab220188. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

     

Properties

Images

  • Immunocytochemistry - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)
    Immunocytochemistry - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)

    This image was produced using ab220188, the same clone but in a different formulation.

    Confocal Immunofluorescence image of Raji cells labeling CD86 with ab220188 followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody (ab213045)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody (ab213045)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling CD86 with ab220188 at 4 µg/ml.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220188).
  • Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)
    Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045)
    Anti-CD86 antibody [C86/1146] (ab220188) at 2 µg/ml + Daudi (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 38 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    CD86 is observed as a 70-kDa glycoprotein in Western blot.

    This image was produced using ab220188, the same clone but in a different formulation.

     

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