Anti-6X His tag® antibody [RMH01] (ab219465)
![Anti-6X His tag® antibody [RMH01] (ab219465)](https://www.abcam.com/ps/products/219/ab219465/Images/ab219465-277020-anti-6x-his-tag-antibody-rmh101-sandwich-elisa.jpg "Anti-6X His tag® antibody [RMH01] (ab219465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RMH01] to 6X His tag®
- Suitable for: ELISA, Sandwich ELISA, WB
- Reacts with: Species independent
Overview
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Product name
Anti-6X His tag® antibody [RMH01]
See all 6X His tag® primary antibodies -
Description
Human monoclonal [RMH01] to 6X His tag® -
Host species
Human -
Specificity
ab219465 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
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Tested applications
Suitable for: ELISA, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide (His-tag). (Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus).
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Positive control
- WB: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (K36M) protein; Sandwich ELISA: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein; purified HisTag-PCSK9 recombinant protein
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General notes
ab219465 is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (ab200537), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab219465 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMH01 -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA - Anti-6X His tag® antibody [RMH101] (ab219465)
Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50µL/well of different concentrations of ab219465 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
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![ELISA - Anti-6X His tag® antibody [RMH101] (ab219465)](https://www.abcam.com/ps/products/219/ab219465/Images/ab219465-277019-anti-6x-his-tag-antibody-rmh101-elisa.jpg)
ELISA - Anti-6X His tag® antibody [RMH101] (ab219465)A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of ab219465 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody
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![Western blot - Anti-6X His tag® antibody [RMH101] (ab219465)](https://www.abcam.com/ps/products/219/ab219465/Images/ab219465-277018-anti-6x-his-tag-antibody-rmh101-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [RMH101] (ab219465)All lanes : Anti-6X His tag® antibody [RMH01] (ab219465) at 0.2 µg/ml
Lane 1 : 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Lane 2 : 293T cells untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Secondary
All lanes : HRP conjugated anti-Human IgG secondary antibody
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![Anti-6X His tag® antibody [RMH01] (ab219465)](https://www.abcam.com/ps/products/219/ab219465/Images/ab219465-4-benefits-of-recombinant-antibodies.png)
Anti-6X His tag® antibody [RMH01] (ab219465)
