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Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7951] to Nucleolin - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nucleolin antibody [EPR7951] - BSA and Azide free
    See all Nucleolin primary antibodies

  • Description

    Rabbit monoclonal [EPR7951] to Nucleolin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248709 is the carrier-free version of ab134164. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248709 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/10000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : Nucleolin knockout HeLa whole cell lysate
    Lane 3 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 76 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab134164, the same antibody clone in a different buffer formulation.

    Lanes 1 - 3: Merged signal (red and green). Green - ab134164 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab134164 was shown to specifically react with Nucleolin in wild-type HeLa cells as signal was lost in Nucleolin knockout cells. Wild-type and Nucleolin knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab134164 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    Western blot - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    All lanes : Anti-Nucleolin antibody [EPR7951] (ab134164) at 1/1000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : HuT-78 cell lysates
    Lane 3 : HeLa cell lysates
    Lane 4 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 76 kDa



    This data was developed using ab134164, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    OI-RD Scanning - Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    This data was developed using ab134164, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)
    Anti-Nucleolin antibody [EPR7951] - BSA and Azide free (ab248709)

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