Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22330-75] to FAK (phospho Y925)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-FAK (phospho Y925) antibody [EPR22330-75]
    See all FAK primary antibodies

  • Description

    Rabbit monoclonal [EPR22330-75] to FAK (phospho Y925)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)
    Western blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)
    All lanes : Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Untreated membrane)
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Phosphatase treated membrane)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 119 kDa


    Exposure time: 70 seconds


    FAK can be cleaved at several sites. The molecular weight observed is consistent with what has been described in the literature (PMID: 10545505, 9642276).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)
    Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)

    Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813 at a 1/1000 dilution). Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as secondary antibody at 1/100000 dilution.

    Lane 1: FAK phospho Y925 peptide.

    Lane 2: FAK non-phospho peptide 

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 mins.

  • Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)
    Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)

© 2021 Astana Biomed Group LLP. All rights reserved.