Anti-eIF3A antibody [EPR5819(2)] (ab128996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5819(2)] to eIF3A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-eIF3A antibody [EPR5819(2)]
See all eIF3A primary antibodies -
Description
Rabbit monoclonal [EPR5819(2)] to eIF3A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human eIF3A aa 1100-1200. The exact sequence is proprietary.
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Positive control
- BxPC3, 293T, Jurkat, C6, RAW264.7, PC12 and L6 lysates; Human brain tissue; BxPC3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5819(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-eIF3A antibody [EPR5819(2)] (ab128996) at 1/1000 dilution
Lane 1 : BxPC3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 166 kDa
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ab128996, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128996, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.
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