Anti-beta Actin antibody [M008] (ab253283)
![Anti-beta Actin antibody [M008] (ab253283)](https://www.abcam.com/ps/products/253/ab253283/Images/ab253283-343948-antibetaactinantibodym008-westernblot-human.jpg "Anti-beta Actin antibody [M008] (ab253283)")
Key features and details
- Mouse monoclonal [M008] to beta Actin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-beta Actin antibody [M008]
See all beta Actin primary antibodies -
Description
Mouse monoclonal [M008] to beta Actin -
Host species
Mouse -
Specificity
ab253283 preferentially detects human beta Actin with only weak reactivity toward actins in rat, mouse, or rabbit.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Other Immunogen Type within Human beta Actin. The exact sequence is proprietary. (Proprietary antigen related to human beta actin in MDA-MB-231 breast cancer cell line).
Database link: P60709 -
Positive control
- WB: HUVEC-CS, Jurkat, HeLa and LNCaP cell lysates. ICC/IF: MeWo cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
M008 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-beta Actin antibody [M008] (ab253283)All lanes : Anti-beta Actin antibody [M008] (ab253283) at 1/1000 dilution
Lane 1 : HUVEC-CS cell lysate
Lane 2 : Rabbit spleen fibroblast cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : LNCaP (human prostate cancer cell line) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 6 : F9 (mouse embryonic testicular cancer cell line) cell lysate
Predicted band size: 41 kDaPrimary for 1 hour at room temperature in PBS/5% non-fat milk/0.045 Tween 20.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [M008] (ab253283)](https://www.abcam.com/ps/products/253/ab253283/Images/ab253283-343949-antibetaactinantibodym008-immunocytochemistryimmunofluorescence-MeWo-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [M008] (ab253283)Paraformaldehyde-fixed MeWo (human epidermoid carcinoma cell line) cells stained for beta Actin (red) using ab253283 at 1/50 dilution in ICC/IF. The antibody was detected using goat anti-mouse DyLight® 594.