Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PMCA1 antibody [EPR12029] (ab190355)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12029] to PMCA1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PMCA1 antibody [EPR12029]
    See all PMCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12029] to PMCA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, HeLa, Neuro-2a, U87-MG and HepG2 cell lysates. IHC-P: Human stomach and Human transitional cell carcinoma of bladder tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
    Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
    All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ATP2B1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 139 kDa
    Observed band size: 180-245 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab190355 observed at 180-245 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab190355 Anti-PMCA1 antibody [EPR12029]  was shown to specifically react with PMCA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265561 (knockout cell lysate ab257365) was used.  Wild-type and PMCA1 knockout samples were subjected to SDS-PAGE.  ab190355 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
    Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
    All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 139 kDa
    Observed band size: 130-250 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
    Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)
    All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 139 kDa
    Observed band size: 130-250 kDa why is the actual band size different from the predicted?

  • Anti-PMCA1 antibody [EPR12029] (ab190355)
    Anti-PMCA1 antibody [EPR12029] (ab190355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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