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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF3A antibody (ab86146)

Price and availability

308 236 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-eIF3A antibody (ab86146)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to eIF3A
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-eIF3A antibody
    See all eIF3A primary antibodies
  • Description

    Rabbit polyclonal to eIF3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 860 and 910 of human eIF3A using the numbering given in entry NP_003741.1.

  • Positive control

    • HeLa whole cell lysate (ab150035) 293T whole cell lysate NIH3T3 whole cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Images

  • Western blot - Anti-eIF3A antibody (ab86146)
    Western blot - Anti-eIF3A antibody (ab86146) This image is courtesy of an anonymous Abreview
    Anti-eIF3A antibody (ab86146) at 1/1000 dilution + Mouse pancreatic cancer whole cell lysate at 20 µg

    Secondary
    IRDye® 680LT-conjugated donkey anti-mouse IgG polyclonal at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 167 kDa
    Observed band size: 166 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 minutes

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody (ab86146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody (ab86146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling eIF3A with ab86146 at 1/200 (1µg/ml). Detection: DAB.
  • Western blot - Anti-eIF3A antibody (ab86146)
    Western blot - Anti-eIF3A antibody (ab86146) This image is courtesy of an anonymous Abreview
    Anti-eIF3A antibody (ab86146) at 1/1000 dilution + Human U2OS osteosarcoma whole cell lysate at 20 µg

    Secondary
    IRDye® 800CW conjugated donkey anti-rabbit polyclonal at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 167 kDa
    Observed band size: 166 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-eIF3A antibody (ab86146)
    Western blot - Anti-eIF3A antibody (ab86146)
    All lanes : Anti-eIF3A antibody (ab86146) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 167 kDa


    Exposure time: 3 seconds
  • Immunoprecipitation - Anti-eIF3A antibody (ab86146)
    Immunoprecipitation - Anti-eIF3A antibody (ab86146)
    Detection of Human eIF3A by Immunoprecipitation, using ab86146 at 3µg/mg lysate. Image shows immunoprecipitated eIF3A detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86146 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 3 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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