Anti-eIF3A antibody (ab86146)
Key features and details
- Rabbit polyclonal to eIF3A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF3A antibody
See all eIF3A primary antibodies -
Description
Rabbit polyclonal to eIF3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
A region between residue 860 and 910 of human eIF3A using the numbering given in entry NP_003741.1.
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Positive control
- HeLa whole cell lysate (ab150035) 293T whole cell lysate NIH3T3 whole cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-eIF3A antibody (ab86146) at 1/1000 dilution + Mouse pancreatic cancer whole cell lysate at 20 µg
Secondary
IRDye® 680LT-conjugated donkey anti-mouse IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 167 kDa
Observed band size: 166 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling eIF3A with ab86146 at 1/200 (1µg/ml). Detection: DAB.
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Anti-eIF3A antibody (ab86146) at 1/1000 dilution + Human U2OS osteosarcoma whole cell lysate at 20 µg
Secondary
IRDye® 800CW conjugated donkey anti-rabbit polyclonal at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 167 kDa
Observed band size: 166 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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All lanes : Anti-eIF3A antibody (ab86146) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 167 kDa
Exposure time: 3 seconds
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Detection of Human eIF3A by Immunoprecipitation, using ab86146 at 3µg/mg lysate. Image shows immunoprecipitated eIF3A detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86146 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 3 seconds.