Anti-Prickle antibody (ab15577)
Key features and details
- Rabbit polyclonal to Prickle
- Suitable for: IHC-P, ELISA
- Isotype: IgG
Overview
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Product name
Anti-Prickle antibody -
Description
Rabbit polyclonal to PrickleThis product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Specificity
Immunogen sequence most homologous with Prickle 1, but is ~65% identical with Prickle 2 sequences, thus some reaction with Prickle 2 may be seen as well as with Prickle 1. -
Species reactivity
Predicted to work with: Mouse, Rat, Human, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Xenopus laevis Prickle aa 300-400 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab15578)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
Application Abreviews Notes IHC-P (2) 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.ELISA Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578).Notes IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.ELISA
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578).Target
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Relevance
Prickle is a LIM domain protein that appears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors. The human protein is a 831 amino acid protein containing 3 N-terminal LIM domains and 3 C-terminal nuclear localization signals. It also contains 4 N-glycosylation sites, 2 PKA phosphorylation sites, and a C-terminal CIIS prenylation motif. -
Cellular localization
Nuclear Membrane -
Database links
- Entrez Gene: 144165 Human
- Entrez Gene: 106042 Mouse
- Entrez Gene: 315259 Rat
- Entrez Gene: 368249 Zebrafish
- Omim: 608500 Human
- SwissProt: Q14C83 Human
- SwissProt: Q96MT3 Human
- SwissProt: Q3U5C7 Mouse
see all -
Alternative names
- FLJ31627 antibody
- FLJ31937 antibody
- MGC138902 antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prickle antibody (ab15577)Image courtesy of an Abreview submitted by Antibody Solutionsab15577 (1/50) staining Prickle in paraffin-embbeded human uterus (smooth muscle). Tissues underwent formaldehyde fixation, heat mediated antigen retrieval and peroxidase blocking prior to staining of the sections. Please refer to Abreview for further experimental details.
Datasheets and documents
References (3)
ab15577 has been referenced in 3 publications.
- Kotrbová A et al. WNT signaling inducing activity in ascites predicts poor outcome in ovarian cancer. Theranostics 10:537-552 (2020). PubMed: 31903136
- Wan Y et al. Prickle1 regulates differentiation of frontal bone osteoblasts. Sci Rep 8:18021 (2018). PubMed: 30575813
- Song H et al. Androgen receptor drives hepatocellular carcinogenesis by activating enhancer of zeste homolog 2-mediated Wnt/ß-catenin signaling. EBioMedicine 35:155-166 (2018). PubMed: 30150059
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prickle antibody (ab15577) Image courtesy of an Abreview submitted by Antibody Solutionsab15577 (1/50) staining Prickle in paraffin-embbeded human uterus (smooth muscle). Tissues underwent formaldehyde fixation, heat mediated antigen retrieval and peroxidase blocking prior to staining of the sections. Please refer to Abreview for further experimental details.