Anti-RAP55 antibody (ab229277)
Key features and details
- Rabbit polyclonal to RAP55
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RAP55 antibody
See all RAP55 primary antibodies -
Description
Rabbit polyclonal to RAP55 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human RAP55 (internal sequence). The exact sequence is proprietary.
Database link: Q8ND56 -
Positive control
- WB: NTERA-2 cl.D1 [NT2/D1] , A549, NCI-H1299 and HCT 116 whole cell extracts. IHC-P: Mouse hindbrain tissue; rat middle brain tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RAP55 antibody (ab229277)
4% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RAP55 (green) using ab229277 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Alpha Tubulin , a cytoskeleton marker, is detected with an alpha Tubulin antibody (red).
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Western blot - Anti-RAP55 antibody (ab229277)All lanes : Anti-RAP55 antibody (ab229277) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-RAP55 antibody (ab229277)Anti-RAP55 antibody (ab229277) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (ab229277)Paraffin-embedded mouse hindbrain stained for RAP55 with ab229277 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55 antibody (ab229277)Paraffin-embedded rat middle brain stained for RAP55 with ab229277 at 1/500 dilution in immunohistochemical analysis.
