Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 10, 2021

Anti-SNX5 antibody [EPR14358] (ab180520)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14358] to SNX5
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-SNX5 antibody [EPR14358]
See all SNX5 primary antibodies
Description

Rabbit monoclonal [EPR14358] to SNX5
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human kidney tissue; Jurkat cells; Jurkat, HepG2 and 293T cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14358
Isotype

IgG
Research areas

Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)

All lanes : Anti-SNX5 antibody [EPR14358] (ab180520) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNX5 with ab180520 at 1/50 dilution. Anti-rabbit HRP secondary antbody was used and cells counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)

Immunofluoresent analysis of Jurkat cells (acetone-fixed) labeling SNX5 with ab180520 at 1/100 dilution. Goat anti rabbit IgG (Dylight 488) secondary antibody was used at 1/250 dilution and cells were counterstained with DAPI.
Anti-SNX5 antibody [EPR14358] (ab180520)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com