Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [NA24] to Nucleophosmin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nucleophosmin antibody [NA24] - BSA and Azide free
    See all Nucleophosmin primary antibodies

  • Description

    Mouse monoclonal [NA24] to Nucleophosmin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human pancreas and liver tissue; Mouse liver and stomach tissue; rat liver and colon tissue. ICC/IF: HeLa cells. Flow cyt: 3T3-L1 and HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256124 is the carrier-free version of ab238029. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    NA24

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) . Nuclear staining on human pancreas. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Nucleophosmin with ab238029 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/2000 diluton (Red). The Nuclear counterstain was DAPI (Blue).

    The negative controls are as follows:
    -ve control 1: ab238029 at 1/100 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human liver. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Flow Cytometry - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Flow Cytometry - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Nucleophosmin with ab238029 at 1/500 dilution (Red) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) . Nuclear staining on mouse liver is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Flow Cytometry - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Flow Cytometry - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 3T3-L1 (Mouse embryonic fibroblast) cells labelling Nucleophosmin with ab238029 at 1/500 dilution (Red) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse stomach is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat liver is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Nucleophosmin with ab238029 at 1/100 dilution (4.76 μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat colon is observed. The section was incubated with ab238029 overnight at 4 ℃. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238029).

  • Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)
    Anti-Nucleophosmin antibody [NA24] - BSA and Azide free (ab256124)

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