Rat EGF ELISA Kit (ab193734)
Key features and details
- Sensitivity: 0.8 pg/ml
- Range: 0.8 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat EGF ELISA Kit
See all EGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.8 pg/ml -
Range
0.8 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 88.98 68% - 110% Plasma = 104.6 69% - 135% Cell culture media = 98.21 81% - 113% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s EGF Rat ELISA Kit (ab193734) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat EGF in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for rat EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures EGF present in the samples. The wells are washed and biotinylated anti-rat EGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of EGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200ml 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated rat EGF detection antibody (lyophilized) 2 vials Pre-coated rat EGF Microplate (12 strips x 8 wells) 12 x 8 tests rat EGF standards (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate. -
Involvement in disease
Hypomagnesemia 4 -
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
Post-translational
modificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
Database links
- Entrez Gene: 25313 Rat
- SwissProt: P07522 Rat
- Unigene: 6075 Rat
Images
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Standard curve - diluent A
Representative Standard curve - using ab193734 Rat EGF standards in diluent A
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Standard curve - diluent BRepresentative Standard curve - using ab193734 Rat EGF standards in diluent B
