Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGF antibody [EPR21926] (ab218831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21926] to EGF
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Rat

Overview

  • Product name

    Anti-EGF antibody [EPR21926]
    See all EGF primary antibodies

  • Description

    Rabbit monoclonal [EPR21926] to EGF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    IP
    Rat
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat EGF active protein (aa974-1026), rat salivary gland tissue lysate and rat urine. IHC-P: Rat kidney tissue. IHC-Fr: Rat kidney tissue. IP: Rat salivary gland tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR21926] (ab218831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR21926] (ab218831)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling EGF with ab218831 at 1/10,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in distal convoluted tubules of rat kidney (PMID 1930151) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-EGF antibody [EPR21926] (ab218831)
    Western blot - Anti-EGF antibody [EPR21926] (ab218831)
    All lanes : Anti-EGF antibody [EPR21926] (ab218831) at 1/5000 dilution

    Lane 1 : Rat salivary gland tissue lysate
    Lane 2 : Rat urine

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    Exposure: Lane 1: 3 minutes; Lane 2: 75 seconds

    The molecular mass observed is consistent with what has been described in the literature (PMID:2268351).

  • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR21926] (ab218831)
    Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR21926] (ab218831)

    Immunohistochemical analysis of frozen rat kidney tissue labeling EGF with ab218831 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on distal convoluted tubule of rat kidney (PMID: 23143303) is observed. Tissue was fixed in 4% PFA and permeabilized using 0.2% Triton X-100.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-EGF antibody [EPR21926] (ab218831)
    Immunoprecipitation - Anti-EGF antibody [EPR21926] (ab218831)

    EGF was immunoprecipitated from 0.35 mg of rat salivary gland lysate with ab218831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218831 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Rat salivary gland tissue 10 µg (Input). 

    Lane 2: ab218831 IP in rat salivary gland lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218831 in rat salivary gland lysate.

    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.

  • Western blot - Anti-EGF antibody [EPR21926] (ab218831)
    Western blot - Anti-EGF antibody [EPR21926] (ab218831)
    Anti-EGF antibody [EPR21926] (ab218831) at 1/1000 dilution + Rat EGF active protein (aa974-1026), 20ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    The molecular mass observed is consistent with what has been described in the literature (PMID:2268351).

  • Anti-EGF antibody [EPR21926] (ab218831)
    Anti-EGF antibody [EPR21926] (ab218831)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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