Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [7B9] to beta II Tubulin - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta II Tubulin antibody [7B9] - BSA and Azide free
    See all beta II Tubulin primary antibodies

  • Description

    Mouse monoclonal [7B9] to beta II Tubulin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat brain tissue lysate. U-87 MG, C6, PC-12, Y79 and MDA-MB-231 whole cell lysate. ICC/IF: U-87 MG, Neuro-2a and PC-12 cells. IP: Neuro-2a whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255982 is the carrier-free version of ab28035. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Human heart (PMID:20191564).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-87 MG cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

    Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)

    beta II Tubulin was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10µg with ab28035 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab28035 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

    Lane 1: Neuro-2a whole cell lysate 10µg.

    Lane 2: ab28035 IP in Neuro-2a whole cell lysate. 

    Lane 3: Mouse monoclonal IgG (ab18443) instead of ab28035 in Neuro-2a whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

    Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : Y79 (human retinoblastoma retinoblastoma), whole cell lysate
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 180 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

    Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

  • Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)
    Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (ab255982)

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