Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
![Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-211355-ab180595wb1.jpg "Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker
See all gamma Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Hela, Jurkat, A431 or 293 lysate, Human colon tissue or Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13872 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)All lanes : Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595) at 1/2000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lane 3 : A431 lysate
Lane 4 : 293 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-211356-ab180595ihc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-211357-ab180595fc1.jpg)
Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)Flow cytometry analysis of Jurkat cells using ab180595 at a 1/140 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
-
![Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-4-benefits-of-recombinant-antibodies.png)
Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
