Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

Key features and details

  • Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster
  • Isotype: IgG1

Overview

  • Product name

    Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker
    See all gamma Tubulin primary antibodies

  • Description

    Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Chicken
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Images

  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    All lanes : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution

    Lane 1 : HeLa cell line lysates
    Lane 2 : U87 cell line lysates
    Lane 3 : COS7 cell line lysates
    Lane 4 : P19 cell line lysates
    Lane 5 : Hepa1-6 cell line lysates
    Lane 6 : CHO cell line lysates
    Lane 7 : MDBK cell line lysates
    Lane 8 : MDCK cell line lysates

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 48 kDa

  • Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

    This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

    See Abreview

  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Lane 1 : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution
    Lane 2 : Negative control (secondary antibody only)

    All lanes : Whole cell extract of chicken fibroblasts

    Secondary
    All lanes : Alkaline Phosphatase conjugated goat anti-mouse IgG

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)
    Immunocytochemistry - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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